Journal: Medical Oncology (Northwood, London, England)

Article Title: Therapeutic exploration of combined local hyperthermia and anti-CD200 blockade therapy in the EMT6 breast cancer model

doi: 10.1007/s12032-026-03240-7

Figure Lengend Snippet: HT combined with anti-CD200 blockade enhances treatment efficacy. A Treatment timeline for HT and anti-CD200 administration in tumor-bearing mice. HT was applied on days 7, 8, and 9 post-inoculation, while anti-CD200 mAb (100 µg/ml, 0.1 ml, i.p.) was given on days 7, 9, and 11 in the combination group. B Individual tumor growth profile for control, hyperthermia, anti-CD200 blockade, and combined groups. C Time to fast tumor growth phase (day). D Mean tumor growth curves (± SEM). E Kaplan Meier survival curves. n = 10 or 11 per group. Statistical comparisons in terms of mean were obtained using Mann-Whitney test, while Mantel-Cox log-rank analysis was used to analyse Kaplan-Meier curves. * P < 0.05, ** P < 0.005, *** P < 0.0005, **** P < 0.0001

Article Snippet: For the CD200 blockade, mice were injected with 100 μg anti-CD200 mAb (clone OX-90, BioXCell) in 100 μl PBS intraperitoneally (i.p.) on day 7, 9 and 11 post-tumor inoculation [ , ].

Techniques: Control, MANN-WHITNEY

Journal: Medical Oncology (Northwood, London, England)

Article Title: Therapeutic exploration of combined local hyperthermia and anti-CD200 blockade therapy in the EMT6 breast cancer model

doi: 10.1007/s12032-026-03240-7

Figure Lengend Snippet: Anti-CD200 blockade therapy reduces infiltrated MDSCs. A Schematic timeline for MDSCs tumor-infiltration analysis. Tumor were harvested at day 21 post-inoculation and analysed by flow cytometry. B Dot plot showing the gating strategy of MDSCs. Cells were gated based on forward and side scatter profile and of those, leukocytes (CD45 + cells) were analysed for MDSCs. Bar graph showing the percentage (mean ± SEM) of leukocytes C and CD11b + Gr1 + cells gated on leukocytes D . n = 4 or 5 per group. ns -non-significant ( P > 0.05), ** P < 0.005, *** P < 0.0005, **** P < 0.0001 (Student’s t -test)

Article Snippet: For the CD200 blockade, mice were injected with 100 μg anti-CD200 mAb (clone OX-90, BioXCell) in 100 μl PBS intraperitoneally (i.p.) on day 7, 9 and 11 post-tumor inoculation [ , ].

Techniques: Flow Cytometry

Journal: Medical Oncology (Northwood, London, England)

Article Title: Therapeutic exploration of combined local hyperthermia and anti-CD200 blockade therapy in the EMT6 breast cancer model

doi: 10.1007/s12032-026-03240-7

Figure Lengend Snippet: Proposed illustration summarizing the effect of local HT treatment combined with anti-CD200 blockade therapy on TME

Article Snippet: For the CD200 blockade, mice were injected with 100 μg anti-CD200 mAb (clone OX-90, BioXCell) in 100 μl PBS intraperitoneally (i.p.) on day 7, 9 and 11 post-tumor inoculation [ , ].

Techniques: